Studies on Whole Cell Fluorescence-Based Screening for Epoxide Hydrolases and Baeyer- Villiger Monoxygenases
نویسندگان
چکیده
Reações de biocatálise foram realizadas em microplacas (200 μL) visando a utilização de substratos fluorogênicos (100 μmol L) para prospecção rápida de epóxido hidrolases (EHs) e Baeyer-Villiger monoxigenases (BVMOs) em microrganismos (células inteiras). Um protocolo final foi alcançado para EHs, com a detecção de 3 novas fontes enzimáticas (Agrobacterium tumefaciens, Pichia stipitis, Trichosporom cutaneum). O ensaio fluorogênico para BVMO não ocorreu como esperado. A abordagem de algumas variáveis envolvidas (aeração; pH) proporcionou a detecção inédita da atividade enzimática de BVM em T. cutaneum.
منابع مشابه
Enhancement of Soluble Expression and Biochemical Characterization of Two Epoxide Hydrolases from Bacillus
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